Octanoylated-lipoyl domain production in YS25 and YS47.

<p>(A) The production of the octanoylated-lipoyl domain under different culture conditions. ‘9h’ indicates 10 μM IPTG plus 1 mM OA were added, and cells were cultured for 9 hours; ‘3h/4h’ indicates cells were cultured for 3 hours and then 4 hours in the presence of 1 mM IPTG plus 1 mM OA; ‘3h/5h’ indicates cells were cultured for 3 hours and then 5 hours in the presence of 1 mM IPTG plus 1 mM OA; ‘6h/3h’ indicates cells were cultured for 6 hours and then 3 hours in the presence of 1 mM IPTG plus 1 mM OA; ‘6h/4h’ indicates cells were cultured for 6 hours and then 4 hours in the presence of 1 mM IPTG plus 1 mM OA. LB medium was used. (B) The production of the octanoylated-lipoyl domain in the presence of various concentrations of OA. YS25 and YS47 were cultured for 6 hours in LB medium and then 3 hours in the presence of 1 mM IPTG plus indicated concentrations of OA. Yield of the octanoylated—lipoyl domain were measured as described in Materials and Methods. Data from three independent experiments are expressed as mean ± S. D.