<p>Indicated strains were cultured in LB medium to OD<sub>600</sub> = 0.3, and the expression of the apo-lipoyl domain was induced for 3 hours. A dash (-) indicates that neither IPTG nor arabinose was added, while a plus sign (+) indicates IPTG (TM245, YS17 and YS28) or arabinose (YS19) was added for induction. The lipoyl domain was analyzed as described in the Materials and Methods section. The gel assay was conducted using non-denaturing polyacrylamide gel electrophoresis.</p
<p>(A) FLAG-PLAT1 was purified using the ANTI-FLAG M2 Affinity Gel and 3X FLAG peptide to remove the...
<p>(A) Expression and purification of recombinant NapA. Lane 1: Total protein of <i>E. coli</i> with...
<p>(A) AlgU<sup>A61V</sup> retained the function of inducing alginate production. The wild type <i>a...
<p>(A) The production of the octanoylated-lipoyl domain under different culture conditions. ‘9h’ ind...
<p>(A) Growth assay of <i>E. coli</i> cells expressing Lpa<sub>t471</sub> (closed triangles) and Lpa...
AbstractA sub-gene encoding the lipoyl domain (residues 1–85) of the lipoate acetyltransferase chain...
<p>(A) Growth assay of <i>E. coli</i> BL21 cells expressing Lpa (open triangles) compared to vector ...
<p>(A.) TM of the parental strain PAK and its derivative <i>lecB</i>::Gm<sup>R</sup> via subsurface ...
Toxin-antitoxin (TA) systems are widespread genetic elements in bacteria thought to contribute to ma...
<p>The LPS samples were separated on SDS-PAGE (<b>A</b>) and SDS-Tricine-PAGE (<b>B</b>) and visuali...
<p>(A) KER176 cells that are auxotrophic for lipoic acid were transformed with plasmids encoding wil...
We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the a...
<p>(A) SDS/PAGE analysis of phosphatidylinositol mannosides (PIMs), lipomannan (LM) and LAM prepared...
<p>SDS-PAGE in which 10 μg of total proteins obtained from a COS cell culture (lane 1), COS cell tra...
<p>[<sup>14</sup>C]-labelled lipoglycan fractions were separated on 15% SDS-PAGE gel from cultures o...
<p>(A) FLAG-PLAT1 was purified using the ANTI-FLAG M2 Affinity Gel and 3X FLAG peptide to remove the...
<p>(A) Expression and purification of recombinant NapA. Lane 1: Total protein of <i>E. coli</i> with...
<p>(A) AlgU<sup>A61V</sup> retained the function of inducing alginate production. The wild type <i>a...
<p>(A) The production of the octanoylated-lipoyl domain under different culture conditions. ‘9h’ ind...
<p>(A) Growth assay of <i>E. coli</i> cells expressing Lpa<sub>t471</sub> (closed triangles) and Lpa...
AbstractA sub-gene encoding the lipoyl domain (residues 1–85) of the lipoate acetyltransferase chain...
<p>(A) Growth assay of <i>E. coli</i> BL21 cells expressing Lpa (open triangles) compared to vector ...
<p>(A.) TM of the parental strain PAK and its derivative <i>lecB</i>::Gm<sup>R</sup> via subsurface ...
Toxin-antitoxin (TA) systems are widespread genetic elements in bacteria thought to contribute to ma...
<p>The LPS samples were separated on SDS-PAGE (<b>A</b>) and SDS-Tricine-PAGE (<b>B</b>) and visuali...
<p>(A) KER176 cells that are auxotrophic for lipoic acid were transformed with plasmids encoding wil...
We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the a...
<p>(A) SDS/PAGE analysis of phosphatidylinositol mannosides (PIMs), lipomannan (LM) and LAM prepared...
<p>SDS-PAGE in which 10 μg of total proteins obtained from a COS cell culture (lane 1), COS cell tra...
<p>[<sup>14</sup>C]-labelled lipoglycan fractions were separated on 15% SDS-PAGE gel from cultures o...
<p>(A) FLAG-PLAT1 was purified using the ANTI-FLAG M2 Affinity Gel and 3X FLAG peptide to remove the...
<p>(A) Expression and purification of recombinant NapA. Lane 1: Total protein of <i>E. coli</i> with...
<p>(A) AlgU<sup>A61V</sup> retained the function of inducing alginate production. The wild type <i>a...