Epitope mapping and affinity measurement of two monoclonal antibodies (5H6, 3A5) against G core fragment.

<p>(A) Primary structure of G surface glycoprotein of RSV A2 strain and Gcf sequence. (B) Epitope mapping and affinity measurement of mAb (5H6 and 3A5) was performed using ELISA with known B-cell epitopes of the RSV G protein (peptide G/144-159, G/164-176, G/174-187, and G/190-204). The 96-well plates were coated with 200 ng or 100 ng of peptide corresponding to each epitope of the antibody. The wells were incubated with the indicated dilutions of mAbs.