Effect of ST258 KP strains on NLRP3 in inflammasome activation.

<p><b>Panel A/B: Effect of ST258-KP strains on pro-IL-1</b>β <b>cleavage and caspase-1 activation.</b> Monocytes or MDC from 3 different donors were cultured at 2x10⁶/ml with live ST258 KP strains for 7 hours at 1:1 cell ratio. Cells were lysed and immunoblotted with antibodies specific to IL-1β and caspase-1 (p20). Results from one representative experiment out of three performed are shown. Histograms show the results of densitometric analysis (mean ± SE) from three different experiments. <b>Panel C: Effect of glibenclamide on cytokine production by monocytes or MDC.</b> Monocytes or MDC from four different donors were cultured at 10⁶ cells/ml with live bacterial cells at 1:1 ratio in the presence (grey columns) or absence (white columns) of 100 μM glibenclamide. Cytokines were measured by Immunoplex array in conditioned media collected after 7 hours of culture.