Brain slice images obtained by color DIRI.

<p>(a) Brightfield image taken with PlanApon 2× (NA 0.08) using the VC50 color camera. The exposure time is 4.6 ms. (b) Darkfield image taken with DIRI with Orca R2 B/W camera. The exposure time is 100 ms. (c) LED-illuminated fluorescence image of area Z1 taken with UPlanSapo40× (NA 0.95) with Orca R2 B/W camera. The exposure time is 100 ms. (d) Epi-fluorescence image of area Z1 taken with UPlanSapo40× (NA 0.95) with Orca R2 B/W camera. The exposure time is 100 ms. (e) LED-illuminated fluorescence image of area Z2 taken with the UPLSAPO 40× silicon immersion lens (NA 1.25) with Orca R2 B/W camera. The exposure time is 100 ms. (f) Epi-fluorescence image of area Z2 taken with the UPLSAPO 40× silicon immersion lens (NA 1.25) with Orca R2 B/W camera. The exposure time is 100 ms. Scale bars in the Figs represent (a–d) 2 mm and (e, f) 0.1 mm.