MOESM8 of A new enzyme-linked immunosorbent assay (ELISA) for human free and bound kallikrein 9

Additional file 8: Figure S4. Development of hybrid ELISAs for the detection of KLK9 heterocomplexes with serine protease inhibitors. In-house generated mAb 28ED436 was used as capture Ab, while pAbs against common serine protease inhibitors (e.g. A1AT, ACT, ATBIII, and A2AP) were used as secondary Abs in two biological fluids (sweat 14 and 15). The A2AP (HRP-conjugated) levels were undetectable and are not shown in the graph. KLK9 measurements were depicted as arbitrary fluorescence units. The ELISAs used include the first antibody as coating and the second antibody for detection. Note detection of free KLK9 in 2 sweat samples (↓). For more discussion see text