Quantitative RT-PCR of a few selected genes, evaluated in RT-PCR analyses.

<p>Quantitative RT-PCR (qRT-PCR) was performed for a few selected virulence genes using gene specific primers to validate semi-quantitative RT-PCR data. (A) <i>g10929</i>, (B) <i>g13754</i>, (C) <i>g11449</i>, (D) <i>g8479</i>, (E) <i>g8640</i>, (F) <i>g12211</i>, (G) <i>g6063</i> and (H) <i>g8642</i>. The <i>Fusarium GAPDH</i> gene was used as an internal control. Relative gene expression levels were determined in proportions to levels of corresponding levels of 1 d <i>F</i>. <i>virguliforme-</i>infected samples, which are defined as 1. Error bars represent standard deviation (SD) of two independent biological replicates (n = 6).