Effect of RGS2 deficiency on cytokines and chemokines in BAL fluid of PBS- and HDM-exposed animals.

<p>A, Wild type C57/Bl6 mice were subjected to i.n. instillation of PBS or HDM (25 μg) 3 times/week for 3 weeks. BAL fluid supernatants were collected and cytokine/chemokine levels quantified using a mouse cytokine/chemokine multiplex Luminex array. Protein release (pg/ml) is shown (upper panel) ranked according to the relative fold induction by HDM (lower panel). Data (<i>N</i> = 5 for PBS- and 8 for HDM-treated groups) are plotted as means ± SE. Significance was tested between PBS and HDM exposures by Mann Whitney U test. B, Wild type (WT) and <i>Rgs2</i>^-/- (KO) mice were subjected to <i>i</i>.<i>n</i>. exposure to PBS (left panels) or HDM (25 μg) (right panels) 3 times/week prior to Luminex analysis as in A. Data (<i>N</i> = 5 for PBS-treated wild type and <i>Rgs2</i>^-/-, <i>N</i> = 8 for HDM-treated wild type and 9 for <i>Rgs2</i>^-/-) are plotted as means ± SE. Significance was tested between wild type and <i>Rgs2</i>^-/- groups by Mann Whitney U test. * <i>P</i> < 0.05, ** <i>P</i> < 0.01.