Suppressed TLR signaling in the livers of LPS-injected NS5A-transgenic mice.

<p>(A) Phosphorylation levels of ERK, IκB-α, and IRF7 were determined by immunoblotting with phospho-specific antibodies in the livers of NS5A-transgenic (Tg) and non-transgenic (NT) mice injected with LPS. (B) Interaction between IRAK1 and TRAF6 was examined using an immunoprecipitation experiment. Liver tissue lysates of NS5A-transgenic (Tg) and non-transgenic (NT) mice were immunoprecipitated with anti-TRAF6 antibody and the immunoprecipitates were subjected to SDS-PAGE followed by immunoblotting with anti-IRAK1 and anti-TRAF6 antibodies. Whole cell lysates (WCL) were similarly subjected to immunoblotting with anti-IRAK1 and anti-vinculin antibodies.