Representative original images of Masson trichrome stained lung sections and their corresponding automated 2D-reconstructed images according to their respective lung tissue density values.

<p>Original images (A, C, E, G, I) correspond to representative Masson trichrome stained lung sections from saline control (A) and BLM-treated lungs at 0.25 mg/kg (C), 0.50 mg/kg (E), 0.75 mg/kg (G) and 1 mg/kg (I). Reconstructed-2D lung sections of their respective original images were obtained by grading their tissue density values, obtained from thousands micro-tiles, in 20 classes of increasing values. Pseudocolours were then assigned to tissue densities according to their class from light blue (low density values) to yellow (high density values) (K). For each original image of saline control and BLM-treated lungs the value of their Ascroft score (mean ± s.e.m.) was indicated. Regarding their corresponding 2D-reconstructed images the value of their Dm (mean ± s.e.m.) and HDFm was indicated, respectively. Note that high density values visualized in 2D-reconstructed images were focalized in fibrotic regions evidenced in their corresponding Masson trichrome stained lung sections. Scale bars: 1 mm.