Distribution of tissue density frequency and determination of high tissue density frequency (HDFm) in saline control and bleomycin (BLM)-treated lungs.

<p>Lung tissue density was determined from several thousands of micro-tiles covering the whole lung sections of saline control (n = 6) and BLM-treated (n = 12/dosing group) mice. A: Tissue density values were graded in 20 classes of increasing values (mean ± s.e.m) and their frequency per class was expressed in percent (compared to the total number of density values). B: BLM administration induced a dose-dependent increase of HDFm which corresponded to the sum of the percentage of tissue densities from class 12 to 20. ****p<0.0001 was considered statistically significant in comparison to the non BLM-treated control group.