The Crt1/Dun1 pathway of the replication stress checkpoint is activated in <i>dpb2-103</i> cells under MMS or HU treatment.

<p><b>(A)</b> Western-Blot detection of Sml1 degradation. Extracts from WT or <i>dpb2-103</i> yeast cells treated with 0,05% MMS were analyzed. <b>(B)</b> Quantitative RT-PCR analysis of <i>RNR3</i> and <i>HUG1</i> transcripts in yeast cells released from G1-arrest in YNBD (green) or YNBD with 200 mM HU (red). Transcript levels were analyzed after 120 and 240 minutes of growth and normnalized to wild-type G1-synchronized cells.