Transduction of cells with CHIKV Env pseudotyped vectors in the presence of soluble GAGs.

<p>293T (left), CHO-K1 (right), and pgsA-745 (bottom) cells were seeded in 384-well plates and transduced with CHIKV Env-pseudotyped vectors transferring a <i>luciferase</i> gene. Before addition to the cells, the vector particles were incubated with DX or one of the indicated GAGs for 30 minutes at 4°C (DX and GAGs in five 3-fold dilutions, ranging from 500.0 to 6.2 μg/μl). One day after transduction, the luciferase expression of the cells was detected by a luminometer [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005318#pntd.0005318.ref020" target="_blank">20</a>]. The results are given as percentages of the untreated control values. The experiment was carried out twice in triplicate, and one representative triplicate result is shown.