TSA treatment alone improves early developmental efficiency of SCNT embryos.

<p>SCNT embryos at the pronuclear stage were cultured in PZM-3 supplemented with 50 nM TSA for 24 h and embryos cultured in PZM-3 containing DMSO were served as NT control, then TSA and DMSO were washed out and embryos were cultured in fresh PZM-3 medium until day 7. The effect of HDACs inhibition on the proportion of embryos that reached the 2-cell stage within 48 h after activation <b>(A)</b>, proportion of embryos developing to the blastocyst stage (Day 7) <b>(B)</b> and total cell number per blastocyst <b>(C)</b> are shown. The experiment was repeated at least three times with 200 embryos per group. All the percentage data are expressed relative to the number of 1-cell embryos and are shown as mean ± S.E.M. Values with different superscripts across treatments indicate significant differences (<i>P</i> < 0.05).