Proteasome degradation pathway of TC-CPE in N2A cells.

<p>(<b>A</b>) Representative Western blot analysis of CPE protein levels in N2A cells transfected with EV, WT-CPE, TC-CPE, and treated with MG132 or without (Veh). (<b>B</b>) Bar graphs show fold increase (%) CPE protein normalized to actin, in treated versus untreated cells transfected with EV, WT-CPE or TC-CPE. ND: Not detectable. One-way ANOVA (F_(2,15) = 14.16, p <0.001, n = 6) followed by Tukey’s multiple comparison test, *p<0.05 compared to EV. (<b>C</b>) Western blot analysis of secreted CPE protein levels in N2A cells transfected with WT-CPE or TC-CPE, in the presence or absence of MG132. Note that MG132 did not affect the secreted levels of TC-CPE protein in N2A cells. t-test, p > 0.05, n = 4.