Localization of Mafb and Gata3 at postnatal stages.

<p>Cochlear cross sections of postnatal SG at P1 (<b>A</b>), P6 (<b>B</b>), P30 (<b>C</b>) and P40 (<b>D</b>) immunostained against TuJ1 (white), Gata3 (green) and Mafb (red). Each row shows different stages. <b>A</b>−<b>A”‘:</b> At P1, Mafb (red) was co-localized in the nuclei with Gata3 (green) in TuJ1 (white)-positive PANs. <b>B</b>−<b>B”‘:</b> At P6, like P1, Mafb was localized in most of PANs, and strongly detected in Gata3 strongly positive cells (indicated by yellow arrowheads). <b>C</b>−<b>C”‘:</b> At P30, Mafb was co-localized with Gata3 expression like P6. TuJ1 was negative or weakly positive in Gata3 and Mafb double positive cells indicated by yellow arrowheads in <b>C</b>. <b>D</b>−<b>D”‘:</b> At P40, Mafb expression was the same as P30. High magnification view is shown in the inset, indicating there existed two types of Mafb-positive cells: Gata3 and Mafb double positive cells, which have rather small cell nucleus and did not express TuJ1 (yellow arrowhead); and Gata3 negative, Mafb cytoplasmic positive, and TuJ1 positive cells, which have larger cell body (blue arrowhead). Nuclei were stained with DAPI (blue). Scale bars, 20 μm.