Endochondral Ossification Is Accelerated in Cholinesterase-Deficient Mice and in Avian Mesenchymal Micromass Cultures - Fig 4

<p><b>(a, b) Expression of AChE and BChE in diaphysis of WT.</b> Note ChEs were also expressed on parts of perichondrium and periosteum, but were completely absent in epiphyses. <b>(c -l) Major matrix components and GP structure are changed in P0 A</b>^<b>-</b><b>B</b>^<b>-</b> <b>mutant mice.</b> (c-f) DAPI staining to detect specifically cell nuclei in GP of A^-B^- KO and WT mice at P0 at low (c, d) and higher magnification (e, f). Note many brightly stained, mostly apoptotic cells in mutant. White arrows indicate high numbers of abnormally positioned of cells in mutant growth plate. (g-l) Serial longitudinal sections of distal femoral growth plates from newborn WT (g-i) and mutant hind limbs (j-l) were hybridized with Col-II (g, j), Col-X (h, k) and MMP-13 (i, l) riboprobes. Note that in mutant both Col-X and MMMP-13 were strongly reduced, while Col-II was still quite high in epiphysis and in periosteal cells on diaphysis of mutant mouse. n = 5 per each sample.