FRAP fluorescence vs. time.

<p>Representative FRAP curve showing the recovery of fluorescence with time after the photobleach, which ended at t = 0. Red points/line are the experimental data, and the blue points/line are the best fit to the data using Eq (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170414#pone.0170414.e002" target="_blank">2</a>). These data are from a noncancerous cell in the cytoplasm. Inset: Same data and fit shown for the first 10 seconds to better show the details during recovery. For the full curve, note that only the 1200 points starting at t = 0 and after were corrected for photobleaching in order to perform the FRAP fit. Thus the first 300 points before the bleach at t = 0 exhibit photobleaching.