Loss of IL-27 signaling results in increased disease severity and lung immunopathology after SeV.

<p><b>(A)</b> Female mice were infected intranasally with SeV and weighed every two days until 17 dpi. Weights are graphed as percent of starting weight. Plotted weights are of two mice per group (mock) and five mice per group (infected) and are representative of weight trends in five separate experiments <i>(p<0</i>.<i>0001)</i>. <b>(B)</b> Survival of infected males after SeV. Findings are pooled from five experiments and include WT (n = 25) and IL-27Rα^-/- (n = 18) mice <i>(p</i> = <i>0</i>.<i>0009</i>, Mantel-Cox test) <b>(C)</b> Whole lung tissue from infected females was homogenized, and viral load was quantified by titration of infected cells and RT-PCR at 3 and 10 dpi. Data are representative of two separate experiments, three to five mice per group. <b>(D)</b> Sections of lung tissue from WT and IL-27Rα^-/- mice were preserved in formalin and stained by hematoxylin and eosin (H&E) at 10 dpi. Mock indicates intranasal inoculation with PBS (vehicle) <b>(E)</b> Lung pathology was scored for severity of inflammation. Histological scores are pooled from three separate experiments with three to five mice per group (*<i>p<0</i>.<i>0155</i>, Mann-Whitney test).