Generation of transgenic tobacco expressing the full-length or the signal peptide-like segment of hpaXm.

<p><b>a)</b> The transformation unit <i>pBI-HpaXm</i> or <i>pBI-HpaXmΔLP</i> constructed in the vector pBI121. Labels on the bottom include the kanamycin-resistant gene <i>NPT II</i>, the <i>35S</i> promoter, and target gene the full-length <i>hpaXm</i> (420 bp) or the N-terminal leader peptide (1-MNSLNTQIGANSSFL-15) deletion mutant <i>hpaXmΔLP</i> (357 bp). Those on top are restriction sites <i>Xba</i>I and X<i>ma</i>I. <b>b)</b> Morphological comparison among T₁ plants of the <i>hpaXm</i>-expressing tobacco (T₁-101-3), the <i>hpaXmΔLP</i>-expressing tobacco (T₁-103-5), the Vec-expressing tobacco (T₁-V35), and the parent untransformed plants. All the experiments each involving 15 plants gave similar results at least.