<i>In vitro</i> and <i>in vivo</i> effects of MET inhibition on cell proliferation.

<p>(A) Representative images of the three cell lines grown at 60% confluence on coverslips and treated or not with SGX523 (5 μM) for 24 hours. Scale bar, 20μm. The BrdU positive nuclei (red) show the cycling cells, and the Hoechst dye was used to stain all the nuclei of the cells (in blue). (B) Quantification of the proliferation rates as described in (A) (*p <0.04, **p<0.005, ***p<0.0001). (C) Soft agar colony formation in the H1975 derivate cell lines. The graph shows the number of colonies after 3 weeks of growing in the presence or absence of SGX523 (5 μM) (*p <0.001). Representative images of untreated and SGX523 treated colonies are also shown. (D) Graphs showing the fold change in volumes in 16 xenografts tumors (8 coming from mice subjected to vehicle and 8 from SGX523 treatment) coming from the three H1975 cell lines during the 12 days of treatment. Tumor volumes were measured at the indicated times using a calliper and calculated in based of the equation 0.4xAxB^2 (A, the long axis and B, the short axis of the tumor). (E) Quantification of phospho-histone H3 (P-H3) staining in the xenograft derived tissue in the presence or absence of SGX523 (**p<0.05) Representative images are also shown. Bar, 250 nm.