Transgene basal expression and loss of expression by TATA box deletion.

<p>Gene transfer assay results performed using the -0.1(ΔICR1-2)GFP (A) and the +20(ΔTATA)GFP (B) constructs. Structure and conventions are the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170969#pone.0170969.g001" target="_blank">Fig 1</a>. (a) Gastrula stage; (b) Pluteus stage. (A) Merged fluorescence and bright-field images (left) and GFP fluorescence images (right) from microinjected embryos are shown. (B) Triple-merged bright-field, GFP fluorescence and Texas Red fluorescence images (left) proving embryos are microinjected and GFP fluorescence images (right). × 20 magnification. Lv: lateral view; av: animal view.