Additional file 4: Figure S3. of Metalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer

Sorting ADAM12hi and ADAM12med subpopulations of SUM159PT cells. Live cells were stained with anti-ADAM12 antibody and propidium iodide (PI). A population of ~1.5% viable (PI-negative) cells with the highest expression of ADAM12, ADAM12hi, and a population with medium expression levels of ADAM12, ADAM12med, corresponding to the middle ~75% of all viable cells, were sorted. A subsequent post-sort flow cytometry and qRT-PCR analyses confirmed an enrichment of ADAM12hi and ADAM12med cells within sorted populations. (TIF 221 kb