Analysis of <i>borealin-2</i> mRNA expression.

<p><b>A</b>: RT-PCR analysis of <i>borealin-2</i> mRNA expression in rainbow trout tissues. Trout tissues tested include testis, brain, muscle, ovary, stomach, gill, head kidney, small intestine, spleen, heart, and liver. Trout <i>actb</i> gene was used as a control for RNA quality. <b>B</b>: RT-qPCR analysis of <i>borealin-2</i> mRNA expression during ovarian development. Ovarian samples (n = 4) at different developmental stages including late pre- (LPV), early- (EV), mid- (MV), and late-vitellogenesis (LV) were used in the analysis. The quantity of <i>borealin-2</i> mRNA was normalized to the quantity of <i>actb</i> gene. <b>C</b>: RT-qPCR analysis of <i>borealin-2</i> mRNA expression during embryonic development. Embryos (n = 4) were collected at 0, 3, 7.5, 11.5, 13.5, 18, 27, 34 hours (h), 2, 3, 4, 5, 6, 8, 10, 12, 16, and 25 days (d) post-fertilization. The quantity of <i>borealin-2</i> mRNA was normalized to the quantity of <i>18S rRNA</i> gene. Means of the normalized gene expression values for each stage (ovarian or embryonic development) were calculated and expressed as relative fold changes. Different letters indicate significant difference (P<0.05).