Analyses of NKL homeobox genes in T-ALL cell lines.

<p>(A) RQ-PCR analysis of HHEX after siRNA-mediated knockdown in LOUCY. Concomitant reduction of transcript levels indicated that LYL1 (left) and GATA3 (middle) activate the expression of HHEX. Forced expression of TAL1 resulted in slightly increased expression of HHEX (right), indicating an activating input as well. (B) RQ-PCR analysis of HHEX after forced expression of NKL homeobox genes HLX1, NKX2-3 and NKX3-1 in LOUCY (left). RQ-PCR analysis of NKX3-1 after siRNA-mediated knockdown of HHEX in LOUCY (middle). RQ-PCR analysis of NKX3-1 after forced expression of NKL homeobox genes HHEX, HLX1 and NKX2-3 in JURKAT (right). (C) RQ-PCR analysis of BMPR1A and BMPR2 after forced expression of NKL homeobox gene NKX2-3 in LOUCY.