Loss of function of LRP5 resulted in damaged osteogenesis in vitro and in vivo.

<p>(A) The relative absorbance of MC3T3-E1 cells transfected with a serial dilution of siLRP5 in the WST-1-based colorimetric assay. (B) Representative 3D images of bone microstructure in the knee-joints in LRP5 WT and KO mice. (C) Quantification of trabecular bone volume fraction (BV/TV) in the knee-joints in WT or KO mice was determined from the μCT measurement. (D) Relative expression levels of SMAD7 in the bone tissues of mice. * p < 0.05, ** p < 0.01, n = 3/group.