LRRC2 localizes to the mitochondria and is regulated by Pgc-1.

<p>A, HEK293 cells expressing a LRRC2-GFP fusion protein were incubated with MitoTracker Red and then imaged via confocal microscopy (left). Chart displays signal co-occurrence (green, LRRC2; red, MitoTracker Red) along the dashed line in unmerged images. Scale bar = 20μm. B, Approximate positions of potential ERRα binding sites in rat, mouse and human <i>LRRC2</i> genes (top panel), <i>Lrrc2</i> mRNA abundance in C2C12 and H9c2 cells following infection with either a GFP- or Pgc-1α-encoding adenovirus (bottom-left panel), and <i>Lrrc2</i> mRNA abundance in the cardiac-specific inducible Pgc-1α/β double-knockout (DKO) mouse 3 weeks post-tamoxifen (TX) treatment (bottom-right panel). C, Transcript abundance of <i>Lrrc2</i> and control transcripts (<i>36b4</i>, <i>Tbp</i> and <i>Hprt</i>) as well as Pgc-1α/β in C2C12 cells following adenovirus-mediated over-expression of Pgc-1α and Pgc-1α/β. D, Transcript abundance of <i>Lrrc2</i> and control transcripts (<i>36b4</i>, <i>Tbp</i> and <i>Hprt</i>) as well as Pgc-1α/β in hearts of cardiac-specific Pgc-1α/β double knockout (DKO) mice and Cre only control mice. All QPCR data are represented as means ± s.e.m and are derived from three independent experiments. *, <i>P</i>≤0.05.