<p>(A) The capacity of the pressure chamber is 100 cm<sup>3</sup>, the range of use is between 2 MPa and 90 MPa. The custom-made software runs the adjusted or cell-specific preconditioning treatment by controlling hydrostatic pressure build up, holding and coming back to atmospheric pressure and the temperature in the pressure chamber. The biological material is placed into an appropriate cell container (e.g. luer lock syringe) filled with the convenient fluid (this case by system water). After proper closing cell container is merged into the pressure filled with water. (B) Groups of 26-somites or Prim-5 stage embryos were placed on ice in zebrafish system water containing cryoprotectants. Cooling occurred according to the following cooling...
Skeletal system development and remodelling is regulated by several different factors, including hor...
Mammalian embryos are surrounded by an acellular shell, the zona pellucida. Hatching out of the zona...
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently rep...
BACKGROUND:Cryopreservation of zebrafish embryos is still an unsolved problem despite market demand ...
The development of miniaturized devices for studying zebrafish embryos has been limited due to compl...
<p>Zebrafish embryos were maintained at 28°C from fertilization to 96 hpf. Larvae at 96 hpf were exp...
The toxicity of five cryoprotectants – methanol, DMSO, glycerol, ethanediol and sucrose – on differe...
Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the po...
<p>(A) Sketch of zebrafish embryo at selected stage. (B) The image of cells-injected dechorionated z...
Abstract Background Zebrafish (Danio rerio) is a model organism that has emerged as a tool for cance...
Fish embryos represent a class of multicompartmental bio-logical systems that have not been successf...
9 pages, 2 figures, 2 tablesWhile vitrification has become the method of choice for preservation of ...
The synergy obtained by the combination of cryoprotectants is a successful strategy that can be bene...
<p>Four wells were used as temperature control wells (wells A1, B3, C4 and D6) and as such contained...
<p>(A) Hatching percentage of embryos cultured in the chip by continuous exposure at varied flow rat...
Skeletal system development and remodelling is regulated by several different factors, including hor...
Mammalian embryos are surrounded by an acellular shell, the zona pellucida. Hatching out of the zona...
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently rep...
BACKGROUND:Cryopreservation of zebrafish embryos is still an unsolved problem despite market demand ...
The development of miniaturized devices for studying zebrafish embryos has been limited due to compl...
<p>Zebrafish embryos were maintained at 28°C from fertilization to 96 hpf. Larvae at 96 hpf were exp...
The toxicity of five cryoprotectants – methanol, DMSO, glycerol, ethanediol and sucrose – on differe...
Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the po...
<p>(A) Sketch of zebrafish embryo at selected stage. (B) The image of cells-injected dechorionated z...
Abstract Background Zebrafish (Danio rerio) is a model organism that has emerged as a tool for cance...
Fish embryos represent a class of multicompartmental bio-logical systems that have not been successf...
9 pages, 2 figures, 2 tablesWhile vitrification has become the method of choice for preservation of ...
The synergy obtained by the combination of cryoprotectants is a successful strategy that can be bene...
<p>Four wells were used as temperature control wells (wells A1, B3, C4 and D6) and as such contained...
<p>(A) Hatching percentage of embryos cultured in the chip by continuous exposure at varied flow rat...
Skeletal system development and remodelling is regulated by several different factors, including hor...
Mammalian embryos are surrounded by an acellular shell, the zona pellucida. Hatching out of the zona...
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently rep...