Ab417 binds to L1CAM-expressing ICC cells and reduces L1CAM levels at the membrane.

<p>(A) Confocal microscopic analysis of Ab417 internalization. SCK-L1 cells were preincubated with Ab417 (10 μg/mL) for the indicated times. Membrane L1CAM-bound Ab417 was detected using anti-Human IgG(H+L) Cross Adsorbed DyLight 594 (red), and internalized Ab417 was detected using anti-human IgG (Fc-specific)-FITC (green). (B) Choi-CK cells were incubated with Ab417 (10 μg/mL) for the indicated times, and the membrane L1CAM was detected by western blot analysis using A10-A3 and pan-cadherin as a loading control. Control (c) indicates the cells that were incubated for 6 h without Ab417. (C) Confocal microscopic images of L1CAM staining of PBS (up)- or Ab417 (down)-treated Choi-CK tumors. The images were taken at x400 magnification. L1CAM index (D) are represented as a percentage of positively-stained area comparing to total area.