The effects of MMV007839 and MMV000972 on PfFNT-mediated L-[¹⁴C]lactate uptake by <i>Xenopus</i> oocytes expressing native PfFNT or PfFNT^Gly107Ser.

<p>The PfFNT-mediated uptake of L-[¹⁴C]lactate by oocytes expressing native PfFNT (black symbols) or oocytes expressing PfFNT^Gly107Ser (white symbols) was measured in the presence of a range of different concentrations of MMV007839 (A) and MMV000972 (B). The component of transport attributable to PfFNT (or to PfFNT^Gly107Ser) was determined by subtracting the uptake of L-[¹⁴C]lactate into non-injected oocytes (averaged from the values obtained across the full range of inhibitor concentrations tested, noting that the uptake of L-[¹⁴C]lactate into non-injected oocytes was unaffected by either inhibitor; <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006180#ppat.1006180.s006" target="_blank">S4 Fig</a>) from the uptake of L-[¹⁴C]lactate measured (at each inhibitor concentration) into oocytes expressing either native PfFNT or PfFNT^Gly107Ser. In all panels, the PfFNT-mediated uptake of L-[¹⁴C]lactate is expressed as a percentage of that determined for native PfFNT in the absence of MMV compound (0.4% v/v DMSO; solvent control). To enable a visual comparison between the native PfFNT and PfFNT^Gly107Ser datasets, only part of the concentration range used for the latter is shown in the main panels. The insets show the data obtained for PfFNT^Gly107Ser over the full concentration range tested. In all cases, the data are averaged from four independent experiments (using oocytes from different frogs) and are shown ± SEM. The uptake was measured at 27.5°C over 10 min at pH 6.4 using 2.7 μM L-[¹⁴C]lactate and 600 μM unlabelled L-lactate.