Eupatilin reduces microglial proliferation in the post-ischemic brain 3 days after tMCAO challenge.

<p>Mice were challenged with tMCAO and eupatilin (Eup, 10 mg/kg, <i>p</i>.<i>o</i>.) was administered immediately after reperfusion. Effects of eupatilin (Eup) on microglial proliferation were assessed in tMCAO-challenged brain 3 days after reperfusion by double immunofluorescence against BrdU and Iba1. (<b>A</b>) Representative images for BrdU- and Iba1-immunopositive cells in the marginal zone of ischemic brain. Scale bar, 50 μm. (<b>B</b>) Quantification of proliferated microglia by counting the number of BrdU/Iba1-double positive cells. n = 5 per group. ***<i>P</i><0.001 versus vehicle-treated tMCAO (tMCAO+veh).