MOESM4 of Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis

Additional file 4: Figure S3. Plasma levels of PKM2 by ELISA and RPPA. a Determination of PKM2 by ELISA in plasma samples of DM, sIBM and CRL patients. b Upper panel, scheme of printing of the plasma samples from CRL, sIBM, DM and PM patients. One nl of 1:20 diluted samples were spotted in quadruplicate. Black boxed: negative controls of BSA; Magenta boxed: standard curves of HCT116 cells; Brown boxed: positive controls of murine IgGs; Orange boxed: standard curve of PKM2 recombinant protein; Green boxed: samples from control donors (CTR); Blue boxed: samples from sIBM patients; Red boxed: samples from DM patients; Yellow boxed: sample from PM patients. Lower panel, parallel array processed with goat anti-mouse IgGs CF647 as negative control. Please note the lack of cross-reactivity against the human plasma IgGs. c The histogram shows the plasma levels of PKM2 quantified by RPPA assay. The results shown are mean ± S.E.M