L-SBA optimization to circumvent serum interference with luminescence readout.

<p>Bactericidal reaction was tested using <i>C</i>. <i>freundii</i> in the presence of 5% BRS and serial dilutions of HI standard serum. At the end of the SBA reaction, we performed: a. one spin and resuspension of bacterial pellets in PBS; b. one wash; c. two washes in PBS, before measuring luminescence. Average luminescence signals and standard deviations are shown from three independent experiments, for each condition (for some points the error bars are not visible because shorter than the symbols).