Transplanted CMK6_SFF-derived OPCs differentiate into oligodendrocytes <i>in vivo</i>.

<p>Distribution of CMK6_SFF-derived cells transplanted into the corpus callosum (CC) of the cerebral cortex (Cx) in neonatal mice. (A) Sections stained with HuN (green) in the CC and Cx (B). The same section immunostained with MBP (red) and DAPI (blue). (C) Enlarged image of B. The dotted lines show needle insertion locations. Scale bars = 50 μm. (D-G) High-magnification images with boxed region in C. Arrowheads show HuN-positive cells co-stained with MBP. Scale bars = 10 μm. (H-J) Immunostained contralateral cerebral cortex with MBP (red), HuN (green) and DAPI (blue). (K) Immunostained cerebellum sections. Enlarged images of MBP (L), HuN (M), and overlay (N). Spinal cord section (O–Q). Scale bars = 50 μm.