AdV endosomal escape is PPxY dependent.

<p>HeLa cells (A) or U2OS cells (B) were infected with varying amounts of adenoviral vector particles expressing GFP (WT in black, M1 in red) and the percentage of GFP positive cells was determined 24hpi by FACS analysis. (C) U2OS cells expressing Gal3-mCherry (red signal) were infected with WT or M1 viruses and fixed at 30 minutes post infection and stained for AdV (green signal). Note that colocalization (yellow signal) indicates membrane rupture. (D) U2OS cells were infected with fluorescent viruses fixed at different time points and stained for endogenous GAL3. At indicated time points Gal3 positive signals where quantified. The total number of Gal3 punctae is indicated. (E) U2OS cells were infected as in D and cell-associated virus was quantified at indicated time points. (F) Colocalization between Gal3 and AdV signals was quantified and is displayed as percentage colocalization of total AdV signal. **: P<0.01 and errors bars are standard error. (See also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006217#ppat.1006217.s001" target="_blank">S1 Fig</a>).