Molecular kinetic scheme and effects of nifedipine on L-type calcium channel traces.

<p>(<b>A</b>) Representative L-type calcium ion-channel current (output) elicited by voltage steps from -90 to 0 mV (input), first in the absence of nifedipine, and after addition of nifedipine at 100 nM and 1 μM concentrations. (<b>B</b>) Molecular kinetic scheme obtained by applying SYSMOLE to the traces and expected effects of nifedipine on transition rates. (<b>C</b>) Fractional occupancies as a function of time of the closed-channel (black), open-channel (red), and inactivated-channel (blue) states resulting from simulating the molecular kinetic scheme. Simulations were generated with the mean value of the kinetic parameters (N = 3–6 traces per treatment). Kinetic parameter values can be found on <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1005376#pcbi.1005376.t001" target="_blank">Table 1</a>.