Characteristics of DENV1 E F193T and ZIKV E F198T RVPs.

<p><b>(A)</b> We assessed the efficiency of prM cleavage of standard (Std) preparations of WT and F193T DENV1 RVPs by SDS-PAGE and Western blotting of pelleted virions using a mouse prM-reactive mAb (top panel). The level of E protein, as detected using mouse mAb 4G2, was used as a loading control (bottom panel). Data are representative of three independent experiments performed using independent RVP preparations. <b>(B)</b> Representative dose-response neutralization curves for Std DENV1 WT and F193T RVPs against mAb E60. Percent infectivity versus mAb concentration is shown. Error bars indicate the range of infectivity from duplicate infections. <b>(C)</b> Comparison of mean EC50 values of E60 against Std DENV1 WT and F193T RVPs using a paired t-test. Values were obtained from five independent experiments performed in duplicate. Error bars indicate the SEM. <b>(D)</b> Representative intrinsic decay curves of Std DENV1 WT and F193T RVPs. Experiments were performed as described in <b><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006178#ppat.1006178.g004" target="_blank">Fig 4A</a></b>. Percent infectivity versus hours of incubation is shown. Error bars indicate the SEM obtained from triplicate infections of Raji-DC-SIGN-R cells. <b>(E)</b> Comparison of the mean half-life of infectivity of Std DENV1 WT and F193T RVPs using a paired t-test. Values were obtained from five independent experiments performed in triplicate. Error bars indicate the SEM. <b>(F)</b> Intrinsic decay of Std ZIKV WT and F198T RVPs. Experiments were performed as described in <b><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006178#ppat.1006178.g004" target="_blank">Fig 4A</a></b>. Percent infectivity versus hours of incubation is shown. Decay curves shown are representative of three independent experiments. Error bars indicate the SEM obtained from triplicate infections of Raji-DC-SIGN-R cells. <b>(G)</b> Neutralization of Std ZIKV WT and F198T RVPs by mAb E60. Percent infectivity, normalized to levels observed in the absence of antibody, versus mAb concentration is shown. Curves shown are representative of four independent experiments. Error bars indicate the range of infectivity from duplicate infections.