Aberrant RAD-51 staining in <i>jmjd-5</i> mutant animals.

<p>(A) Schematic representation of a <i>C</i>. <i>elegans</i> gonad arm, showing the position of the 7 zones scored for RAD-51 foci. TZ is the transition zone. Red dots represent RAD-51 foci distribution, according to literature. (B) Top. Histograms showing the quantification of RAD-51 foci in the extracted germlines of N2 and <i>jmjd-5(tm3735)</i> animals. Numbers in parenthesis indicate the number of nuclei analyzed. Bottom. Representative images of RAD-51 (red) and DAPI (blue) staining in germlines dissected from N2 and <i>jmjd-5(tm3735)</i> animals grown at 20°C. Nuclei from zones 4, 5, 6 and 7 are shown. (C) Top. Quantification of RAD-51 foci per nucleus in zones 6 and 7 of N2 and <i>jmjd-5(tm3735)</i> germlines, treated or not with IR (30Gy, 6 hours after the IR). At least 40 nuclei, from a minimum of 4 germlines for each genotype, were quantified for each zone and genotype. ****<i>p</i>< 0.0001, **<i>p</i>< 0.001, with two tailed unpaired t-test. Bottom. Representative images of RAD-51 (red) and DAPI (blue) staining in germlines dissected from N2 and <i>jmjd-5</i> animals, 6h after IR (30Gy). Nuclei from zones 6 and 7 are shown. In (B) and (C), each image shows a projection of multiple z-stacks (0.2 μm spacing) of the entire nuclei. 100X magnification, scale bar 2 μm.