Ask
Search
My library
ORKG

ORKG Ask

Our mission is to organize scholarly knowledge and make it accessible to humans and machines.

About

About ORKG Ask
Report issue
Statistics
Contact

Legal

Terms of use
Data protection (sheet)
Imprint
Accessibility
License

Technical

We
open source
System status
Changelog
Frontend: v1.41.0
Backend: v1.13.4

We use cookies to provide a better user experience.

Data Protection

Immunocytochemical staining of endothelial cell lines on cytospin slides for mdr proteins.

Agnieszka Krawczenko (3756535)
Aleksandra Bielawska-Pohl (3756526)
Karolina Wojtowicz (3756523)
Roksana Jura (3756541)
Maria Paprocka (3756529)
Elżbieta Wojdat (3756532)
Urszula Kozłowska (3756538)
Aleksandra Klimczak (3756517)
Catherine Grillon (350812)
Claudine Kieda (350811)
Danuta Duś (3756520)

Publication date

February 2017

DOI

10.1371/journal.pone.0172371.g003

Abstract

<p>LoVo/Dx cell line was used as a positive control. Slides incubated with secondary antibody (rabbit/mouse link) served as a negative control.</p

Extracted data

Related items

LDL binds to the ECM of mouse vascular smooth muscle (MOVAS) cells.

Esmond N. Geh (17879598)
Debi K. Swertfeger (1555513)
Hannah Sexmith (17879601)
Anna Heink (1575568)
Pheruza Tarapore (530130)
John T. Melchior (12065632)
W. Sean Davidson (1555525)
Amy Sanghavi Shah (17879604)

January 2024

Immunofluorescence staining for LDL and chondroitin sulfate in monolayer MOVAS cells. The cells wer...

Christina T. Teng (340576)
Yin Li (117285)
Pat Stockton (340579)
Julie Foley (340580)

February 2013

<p>Paraffin-embedded control mouse kidney sections were immunostained with the P2 for ERRα, pG for E...

Indirect immunofluorescence staining to investigate binding to LMH cells.

Xinquan Zhang (546832)
Ivana Bilic (162631)
Ana Marek (2609935)
Martin Glösmann (353649)
Michael Hess (127238)

April 2016

<p>Cells were incubated with 500nM recombinant proteins, each, for 1h and fixed, then stained with a...

Cells injected in Matrigel are responsible for formation of new blood vessels.

Glenda Parra-Bonilla (463564)
Diego F. Alvarez (463565)
Mikhail Alexeyev (453796)
Audrey Vasauskas (463566)
Troy Stevens (57952)

September 2013

<p>(A) Representative micrographs of GFP-positive endothelial cells. The presence of GFP was detecte...

Expression of E- and N-cadherin in stable L-cell lines.

William R. Taylor (162523)
Rosalind Morley (309613)
Alexey Krasavin (309615)
Lauren Gregory (309617)
David G. Wilkinson (309619)
Alexei Poliakov (309621)

February 2013

<p>Western blots of cell lysates stained with anti-E-cadherin antibodies (A) and anti-N-cadherin ant...

Immunofluorescence showing IgG expression in sarcoma cell lines.

Zhengshan Chen (126192)
Jing Li (10611)
Yanna Xiao (349235)
Junjun Zhang (2035)
Yingying Zhao (349236)
Yuxuan Liu (349237)
Changchun Ma (349239)
Yamei Qiu (349241)
Jin Luo (349243)
Guowei Huang (129576)
Christine Korteweg (126198)
Jiang Gu (126204)

February 2013

<p>A, normal mouse IgG was used instead of the primary antibody (negative control). B to D, the prim...

Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation for another 144 h in monolayer culture with Rabbit Anti-Human Polyclonal Collagen III Primary Antibody (green) and Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (red).

Wan Tai Seet (310327)
Manira Maarof (310328)
Khairoji Khairul Anuar (310329)
Kien-Hui Chua (310330)
Abdul Wahab Ahmad Irfan (310331)
Min Hwei Ng (310332)
Bin Saim Aminuddin (310333)
Bt Hj Idrus Ruszymah (310334)

February 2013

<p>Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation...

Negative and positive controls for immunostaining of VEGF and CD34.

Elnaz Zand (17014804)
Elias Rajablou (17014807)
Seyedeh Fatemeh Siadat (17014810)
Bahare Beiki (17014813)
Vahid Akbarinejad (11092135)
Christiani Andrade Amorim (4530817)
Mojtaba Rezazadeh Valojerdi (5992115)
Leila Alsadat Tahaei (17014816)
Rouhollah Fathi (7349051)

September 2023

For preparation of negative control, the histological slides of ovarian follicles were merely treate...

Stable cell lines express HCMV US27 or US27 mutants.

Carolyn C. Tu (663652)
Juliet V. Spencer (521467)

November 2014

<p>A) Cell lysates were separated by SDS-PAGE and then immunoblotted using anti-FLAG M2 antibody or ...

TMPs from cells exposed to anti-VEGF-A antibody exhibit reduced ability to promote endothelial cell activity.

Michal Munster (586345)
Ella Fremder (586346)
Valeria Miller (586347)
Neta Ben-Tsedek (586348)
Shiri Davidi (586349)
Stefan J. Scherer (586350)
Yuval Shaked (37384)

April 2014

<p>Matrigel plugs containing an equal number of TMPs (0.5×10<sup>6</sup>) from untreated or B20-expo...

CAP350 peripheral labelling is dependent on E-cadherin-mediated cell adhesion.

Maria P. Gavilan (701309)
Marina Arjona (701310)
Angel Zurbano (701311)
Etienne Formstecher (257888)
Juan R. Martinez-Morales (701312)
Michel Bornens (701313)
Rosa M. Rios (701314)

March 2015

<p>(<b>A</b>) Control cells were treated with 4 mM EGTA for 2 h to disrupt cell-cell contacts, fixed...

Endothelial integrity of mLSECs.

Petra Koudelkova (821256)
Gerhard Weber (821257)
Wolfgang Mikulits (30930)

November 2015

<p>(A) Western blot analysis of early and late passaged mLSECs using anti-CD31, anti-VE-cadherin and...

Immunostaining of MCF-7 cell line cells with anti-EpCAM-FITC- together with a) anti-IGF-IR-PE- or b) anti-VEGFR-2-PE-antibodies.

Monika Pizon (283886)
Dorothea Sonja Zimon (283890)
Ulrich Pachmann (283894)
Katharina Pachmann (15479)

February 2013

<p>MCF-7 cell line also expresses green EpCAM staining heterogeniously, similar to CETCs (positive c...

Immunohistochemistry staining for Snail in rat heart tissue sections during FO closure.

Graeme C. Elliott (629040)
Rockesh Gurtu (629041)
Charles McCollum (629042)
William G. Newman (629043)
Tao Wang (12008)

September 2014

<p>Positive signals are brown colour by DAB staining. Scale bars = 50 µm. Negative control: sections...

mMP modifies endothelial junctional protein expression.

Beryl Wen (539743)
Valery Combes (104149)
Amandine Bonhoure (539744)
Babette B. Weksler (539745)
Pierre-Olivier Couraud (104144)
Georges E. R. Grau (104152)

March 2014

<p>After overnight treatment of endothelial cells with mMP, no changes were observed in the levels o...

LDL binds to the ECM of mouse vascular smooth muscle (MOVAS) cells.

Esmond N. Geh (17879598)
Debi K. Swertfeger (1555513)
Hannah Sexmith (17879601)
Anna Heink (1575568)
Pheruza Tarapore (530130)
John T. Melchior (12065632)
W. Sean Davidson (1555525)
Amy Sanghavi Shah (17879604)

January 2024

Immunofluorescence staining for LDL and chondroitin sulfate in monolayer MOVAS cells. The cells wer...

Christina T. Teng (340576)
Yin Li (117285)
Pat Stockton (340579)
Julie Foley (340580)

February 2013

<p>Paraffin-embedded control mouse kidney sections were immunostained with the P2 for ERRα, pG for E...

Indirect immunofluorescence staining to investigate binding to LMH cells.

Xinquan Zhang (546832)
Ivana Bilic (162631)
Ana Marek (2609935)
Martin Glösmann (353649)
Michael Hess (127238)

April 2016

<p>Cells were incubated with 500nM recombinant proteins, each, for 1h and fixed, then stained with a...

Cells injected in Matrigel are responsible for formation of new blood vessels.

Glenda Parra-Bonilla (463564)
Diego F. Alvarez (463565)
Mikhail Alexeyev (453796)
Audrey Vasauskas (463566)
Troy Stevens (57952)

September 2013

<p>(A) Representative micrographs of GFP-positive endothelial cells. The presence of GFP was detecte...

Expression of E- and N-cadherin in stable L-cell lines.

William R. Taylor (162523)
Rosalind Morley (309613)
Alexey Krasavin (309615)
Lauren Gregory (309617)
David G. Wilkinson (309619)
Alexei Poliakov (309621)

February 2013

<p>Western blots of cell lysates stained with anti-E-cadherin antibodies (A) and anti-N-cadherin ant...

Immunofluorescence showing IgG expression in sarcoma cell lines.

Zhengshan Chen (126192)
Jing Li (10611)
Yanna Xiao (349235)
Junjun Zhang (2035)
Yingying Zhao (349236)
Yuxuan Liu (349237)
Changchun Ma (349239)
Yamei Qiu (349241)
Jin Luo (349243)
Guowei Huang (129576)
Christine Korteweg (126198)
Jiang Gu (126204)

February 2013

<p>A, normal mouse IgG was used instead of the primary antibody (negative control). B to D, the prim...

Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation for another 144 h in monolayer culture with Rabbit Anti-Human Polyclonal Collagen III Primary Antibody (green) and Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (red).

Wan Tai Seet (310327)
Manira Maarof (310328)
Khairoji Khairul Anuar (310329)
Kien-Hui Chua (310330)
Abdul Wahab Ahmad Irfan (310331)
Min Hwei Ng (310332)
Bin Saim Aminuddin (310333)
Bt Hj Idrus Ruszymah (310334)

February 2013

<p>Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation...

Negative and positive controls for immunostaining of VEGF and CD34.

Elnaz Zand (17014804)
Elias Rajablou (17014807)
Seyedeh Fatemeh Siadat (17014810)
Bahare Beiki (17014813)
Vahid Akbarinejad (11092135)
Christiani Andrade Amorim (4530817)
Mojtaba Rezazadeh Valojerdi (5992115)
Leila Alsadat Tahaei (17014816)
Rouhollah Fathi (7349051)

September 2023

For preparation of negative control, the histological slides of ovarian follicles were merely treate...

Stable cell lines express HCMV US27 or US27 mutants.

Carolyn C. Tu (663652)
Juliet V. Spencer (521467)

November 2014

<p>A) Cell lysates were separated by SDS-PAGE and then immunoblotted using anti-FLAG M2 antibody or ...

TMPs from cells exposed to anti-VEGF-A antibody exhibit reduced ability to promote endothelial cell activity.

Michal Munster (586345)
Ella Fremder (586346)
Valeria Miller (586347)
Neta Ben-Tsedek (586348)
Shiri Davidi (586349)
Stefan J. Scherer (586350)
Yuval Shaked (37384)

April 2014

<p>Matrigel plugs containing an equal number of TMPs (0.5×10<sup>6</sup>) from untreated or B20-expo...

CAP350 peripheral labelling is dependent on E-cadherin-mediated cell adhesion.

Maria P. Gavilan (701309)
Marina Arjona (701310)
Angel Zurbano (701311)
Etienne Formstecher (257888)
Juan R. Martinez-Morales (701312)
Michel Bornens (701313)
Rosa M. Rios (701314)

March 2015

<p>(<b>A</b>) Control cells were treated with 4 mM EGTA for 2 h to disrupt cell-cell contacts, fixed...

Endothelial integrity of mLSECs.

Petra Koudelkova (821256)
Gerhard Weber (821257)
Wolfgang Mikulits (30930)

November 2015

<p>(A) Western blot analysis of early and late passaged mLSECs using anti-CD31, anti-VE-cadherin and...

Immunostaining of MCF-7 cell line cells with anti-EpCAM-FITC- together with a) anti-IGF-IR-PE- or b) anti-VEGFR-2-PE-antibodies.

Monika Pizon (283886)
Dorothea Sonja Zimon (283890)
Ulrich Pachmann (283894)
Katharina Pachmann (15479)

February 2013

<p>MCF-7 cell line also expresses green EpCAM staining heterogeniously, similar to CETCs (positive c...

Immunohistochemistry staining for Snail in rat heart tissue sections during FO closure.

Graeme C. Elliott (629040)
Rockesh Gurtu (629041)
Charles McCollum (629042)
William G. Newman (629043)
Tao Wang (12008)

September 2014

<p>Positive signals are brown colour by DAB staining. Scale bars = 50 µm. Negative control: sections...

mMP modifies endothelial junctional protein expression.

Beryl Wen (539743)
Valery Combes (104149)
Amandine Bonhoure (539744)
Babette B. Weksler (539745)
Pierre-Olivier Couraud (104144)
Georges E. R. Grau (104152)

March 2014

<p>After overnight treatment of endothelial cells with mMP, no changes were observed in the levels o...

LDL binds to the ECM of mouse vascular smooth muscle (MOVAS) cells.

Esmond N. Geh (17879598)
Debi K. Swertfeger (1555513)
Hannah Sexmith (17879601)
Anna Heink (1575568)
Pheruza Tarapore (530130)
John T. Melchior (12065632)
W. Sean Davidson (1555525)
Amy Sanghavi Shah (17879604)

January 2024

Immunofluorescence staining for LDL and chondroitin sulfate in monolayer MOVAS cells. The cells wer...

Christina T. Teng (340576)
Yin Li (117285)
Pat Stockton (340579)
Julie Foley (340580)

February 2013

<p>Paraffin-embedded control mouse kidney sections were immunostained with the P2 for ERRα, pG for E...

Indirect immunofluorescence staining to investigate binding to LMH cells.

Xinquan Zhang (546832)
Ivana Bilic (162631)
Ana Marek (2609935)
Martin Glösmann (353649)
Michael Hess (127238)

April 2016

<p>Cells were incubated with 500nM recombinant proteins, each, for 1h and fixed, then stained with a...