RT-PCR detection of DGAT1 genes in transgenic yeast (INVSc1). The yeast actin was used as an internal control. 1, The yeast transformed with pYES2.0; 2â5, the yeast expressing AtDGAT1, GmDGAT1, BnDGAT1 and CeDGAT1, respectively. (DOCX 55Â kb
Immunodetection of LiDGAT1 (red), three type 2 LiDGATs (green) and LDs (blue) in H1246 cells complem...
Growth curves of the 31 transformants selected as the best candidates. S. cerevisiae BY4741_haa1Δ (A...
Alignment of amino acid sequences of L. incisa LiDGATs of type 2 with selected animal and plant DGAT...
Comparison of the predicted structures of CeDGAT1, AtDGAT1, GmDGAT1 and BoDGAT1. (DOCX 416Â kb
Representative TLC plates showing activity of LiDGATs expressed in yeast mutants without and with fe...
Table listing DGAT-encoding genes used for phylogenetic and sequence analyses. (PDF 151 kb
Figure S1. Optimization of transformation conditions. Unless indicated otherwise, the same volume (1...
Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic re...
Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 ...
Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy....
Figure S3. T-DNA organizations in contructs used in this study. All binary vectors have the same pPZ...
Raw transcriptomic data on LiDGATs under diverse nitrogen conditions. (XLSX 9 kb
Full-length DGAT2 sequences identified in the selected algal species. (XLSX 10 kb
Alignment of L. incisa LiDGAT1protein sequence with its animal and plant counterparts and identifica...
Phylogenetic analysis of the DGAT2 family in algae and other representative taxa. (TXT 63 kb
Immunodetection of LiDGAT1 (red), three type 2 LiDGATs (green) and LDs (blue) in H1246 cells complem...
Growth curves of the 31 transformants selected as the best candidates. S. cerevisiae BY4741_haa1Δ (A...
Alignment of amino acid sequences of L. incisa LiDGATs of type 2 with selected animal and plant DGAT...
Comparison of the predicted structures of CeDGAT1, AtDGAT1, GmDGAT1 and BoDGAT1. (DOCX 416Â kb
Representative TLC plates showing activity of LiDGATs expressed in yeast mutants without and with fe...
Table listing DGAT-encoding genes used for phylogenetic and sequence analyses. (PDF 151 kb
Figure S1. Optimization of transformation conditions. Unless indicated otherwise, the same volume (1...
Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic re...
Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 ...
Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy....
Figure S3. T-DNA organizations in contructs used in this study. All binary vectors have the same pPZ...
Raw transcriptomic data on LiDGATs under diverse nitrogen conditions. (XLSX 9 kb
Full-length DGAT2 sequences identified in the selected algal species. (XLSX 10 kb
Alignment of L. incisa LiDGAT1protein sequence with its animal and plant counterparts and identifica...
Phylogenetic analysis of the DGAT2 family in algae and other representative taxa. (TXT 63 kb
Immunodetection of LiDGAT1 (red), three type 2 LiDGATs (green) and LDs (blue) in H1246 cells complem...
Growth curves of the 31 transformants selected as the best candidates. S. cerevisiae BY4741_haa1Δ (A...
Alignment of amino acid sequences of L. incisa LiDGATs of type 2 with selected animal and plant DGAT...