Additional file 1: Table S1. of Analyses of the peripheral immunome following multiple administrations of avelumab, a human IgG1 anti-PD-L1 monoclonal antibody

Antibodies used for 5 panel stain to identify 123 peripheral immune cell subsets. Panel 1: PD-1 signaling; Panel 2: CD4+ T cells, CD8+ T cells, B cells; Panel 3: Tregs; Panel 4: NK, NK-T, cDC, pDC; Panel 5: MDSC. Table S2. Peripheral immune cell subsets analyzed by flow cytometry. Analysis of 123 subsets using 30 unique markers. Table S3. PD-L1 clone MIH-1 used to detect surface expression of PD-L1 in immune cell subsets does not compete for binding with avelumab. Table S4. Effect of three cycles of avelumab on classic and PD-L1+ classic immune cell subsets. Classic and PD-L1+ classic subsets were examined pre-therapy and post-three cycles (n = 14) of avelumab. Table S5. Effect of avelumab on PD-L1+ refined immune cell subsets. PD-L1+ refined subsets were examined pre-therapy and post-one cycle (n = 19), three cycles (n = 14), and nine cycles (n = 16) of avelumab. (PDF 464 kb