Immunostaining of the 2-week <i>Wnt1-Cre;Pdpn</i>^<i>Δ/+</i> mouse lower incisor sagittal section for podoplanin (PDPN).

<p>In the left images at the higher magnification of the parts highlighted by box (c) in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171912#pone.0171912.g007" target="_blank">Fig 7</a>, the expression of PDPN is observed in the odontoblasts (Ob), in the inner enamel epithelial cells (IEE), and in the outer enamel epithelial cells (OEE), but not in the pre-odontoblasts (pOb). There is no expression of PDPN observed in the dental pulp fibroblasts (DP). In the right images, by confocal microscopy at a higher magnification of the area of the left images, the expression of PDPN is observed on the cell membrane of odontoblasts (Ob) and inner enamel epithelial cells (IEE). Bar: left 100μm, right 10μm.