<i>Metacoder</i> has an intuitive and easy to use syntax.

<p>The code in this example analysis parses the taxonomic data associated with sequences from the Ribosomal Database Project [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1005404#pcbi.1005404.ref009" target="_blank">9</a>] 16S training set, filters and subsamples the data by sequence and taxon characteristics, conducts digital PCR, and displays the results as a heat tree. All functions in bold are from the <i>metacoder</i> package. Note how columns and functions in the taxmap object (green box) can be referenced within functions as if they were independent variables.