Comparison between NeurA, CST and CBM media.

<p>(A) Brightfield images at 10 DIV. Scale bars, 500 μm. Time course of LDH and lactate concentration in culture supernatant; n = 3 each condition per time point. Error bars indicate SD. Statistical significant differences are indicated for CBM and CST versus NeurA, with **<i>p</i> < 0.01. (B) Representative confocal images of CA1 and CA3 area of NeuN-stained organotypic hippocampal cultures at 10 DIV. Scale bars, 50 μm. Neuron counts in CA1 and CA3 are shown on the right; n = 3 each condition. (C) Representative recordings of electrographic seizure-like events in organotypic cultures on 10 DIV. Seizure detail is shown in lower traces. Red lines indicate the threshold for paroxysmal event detection. (D) Representative recordings on different days in vitro in cultures maintained in NeurA, revealing the time course of epileptogenesis in organotypic hippocampal cultures. (E) Electrographic seizures (ictal events) in NeurA and CBM in electrographic recordings of seizing slices. For 0–3 DIV, 4–7 DIV, 8–10 DIV, 11–14 DIV,n = 17, 9, 8, 10 for NeurA, n = 15, 8, 10, 10 for CBM, respectively. n represents the number of cultures. *<i>p</i> < 0.05. Error bars represent 95% confidence intervals for incidence of ictal activity and SD for other graphs.