ZIKV <i>in vitro</i> infection and genomics.

<p>(<b>A</b>) Vero cells were infected with ZIKV for 72 h and submitted to indirect immunofluorescence with the anti-flaviviral 4G2 monoclonal antibody. Inset: higher magnification of 4G2 staining surrounding the nuclei of infected Vero cells. Red: Zika virus labeling; Blue: Cell nuclei stained with DAPI; scale bars, 0.1 mm. (<b>B</b>) The linear ssRNA (+) (10,640 bp) complete cds (+ partial UTRs) sequence of ZIKV (HS-2015-BA-01), highlighting the 339 bp amplicon of the E protein gene (see DNA bands from five independent samples in the inset), which was cloned and sequenced.