In situ hybridization confirms the expression of Slit1-3 and Robo1-2 in sensory neurons of the DRG.

<p>(A) Sense mRNA probe control, no blue signal has been developed in all the control samples using Slit1-3 and Robo1-2 sense probes for in situ hybridization (Robo1 sense mRNA probe control was shown in A, images for Robo2 and Slit1-3 sense mRNA probe control not shown). A positive signal for Robo1 (B), Robo2 (C), Slit1 (D), Slit2 (E) and Slit3 (F) were observed in sensory neuronal cell bodies. Slit2 (E) and Slit3 (F) signal also could be observed in the nerve in addition to their strong signal in the neuronal cell bodies of the DRG. The in situ hybridization results thus help to further confirm the specificity of the Slit1-3 and Robo1-2 antibodies used for immunohistochemistry.