SFTSV activates host innate immunity in Hela cells. Endogenous IFNα/β were up-regulated by SFTSV in Hela cells but not in Huh7.0 0r Huh7.5.1 cells.

<p>Hela(A), Huh7.0(B) and Huh7.5.1(C) cells were infected with1.0 MOI SFTSV for 2 hours and cultured for 48 hours before RNA were collected with Trizol reagent following the manufacturer’s protocol. The levels of TLR3, RIG-I, IFNα, IFNβ, GAPDH mRNA and SFTSV mRNA were measured by quantitative RT-PCR. (D) Protein levels of IFNα/β were up-regulated in the culture medium of Hela cells stimulated by SFTSV. Hela and Vero cells were infected with SFTSV (MOI = 1.0) for 2 hours and cultured for 48 hours before the culture medium were collected, The protein levels of IFNα/β were assayed by the Elisa Kit. Data were presented as mean± SD (n = 3). *p<0.05,**p<0.01, ***p<0.001.Data are representative of at least 3 repeated experiments.