OTS167 reduces transcript and protein levels of mutant p53.

<p>(A) qPCR reveals that mutant p53 transcript is significantly downregulated in MDA-MB-231, BT-549 and SUM-159 after 48h and 72h (45 nM) OTS167 treatment. (B) Western blots show that mutant p53 protein is significantly reduced in MDA-MB-231, BT-549 and SUM-159, but WT p53 is increased in MCF-7 (48h, 45 nM OTS167, densitometric data from three experiments). (C) p53 protein reduction is independent of cell density (MDA-MB-231, 96h, 45nM OTS167). Significance was calculated using a Student’s two-tailed t-test: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).