Cellular KAP1 regulates EBV lytic cycle in Burkitt Lymphoma cells.

<p><b>A-C.</b> HH514-16 Burkitt lymphoma (BL) cells were transfected with scrambled siRNA (non-targeting control; open bars in B, C) or siRNA to <i>KAP1</i> (black bars in B, C); <b>D-F.</b> BL cells were transfected with empty vector (open bars in E, F) or pFLAG-CMV2-KAP1 (black bars in E, F). Transfected cells were treated with NaB 24 hours after transfection, and harvested at indicated times (A, D), 24 hours (B, E), or 48 hours (C, F) post-treatment to determine relative amounts of ZEBRA, KAP1 and β-actin by immunoblotting (A, D), relative levels of transcripts from EBV lytic genes <i>BZLF1</i>, <i>BMRF1</i> and <i>BFRF3</i> by qRT-PCR after normalization to 18S rRNA using the ΔΔC_T method (B, E), and relative levels of cell-associated EBV DNA by qPCR (C, F). Error bars: SEM of 3 experiments with 4 technical replicates. Western blots are representative of 2 experiments. Numbers below blots indicate relative amounts of protein after normalization to β-actin.