Effect of antibodies against IFN-γ.

<p>HCV-infected human hepatocyte chimeric mice were injected with 4×10⁷ human PBMCs. The mice were then treated with 1000 IU/kg of IFN-α for 7 days. One day before human PBMC transplantation, mice were injected either with isotype (n = 3) or with antibody against IFN-γ (n = 3). (a) Reductions of serum HCV RNA levels and human albumin concentrations are shown. Mice treated with IFN without PBMCs injection were also analyzed (gray circles). Data are presented as the mean ± SD. (b) Liver mononuclear cells isolated from mice were stained with antibodies against human CD45 and mouse CD45, and analyzed by flow cytometry (upper panel). Statistical analysis of the frequencies of human mononuclear cells in PBMC-treated mice are shown in the lower panel. Data are presented as the mean ± SD.